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Conjugated olefin hydroxylation by phanerochaete chrysosporium and horseradish peroxidase
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Conjugated olefin hydroxylation by phanerochaete chrysosporium and horseradish peroxidase
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http://www.ohsu.edu/xd/education/library/services/theses-dissertations/rights-statement.cfm
Title
Conjugated
olefin
hydroxylation
by
phanerochaete
chrysosporium
and
horseradish
peroxidase
Creator.PersonalName
Kuhn
,
Robert
M
.
Thesis.Degree
M.S.
Thesis.Major
Biochemistry
Thesis.DateDegreeAwarded
December
1981
Institution
Oregon Graduate Center
Department
Dept. of Biochemistry
Thesis.Advisor/Mentor
Gold, Michael H.
Thesis.Committee
Hurst, James K.
Hauser, Frank M.
Pankow, James F.
Subject.LCSH
Lignin
Wood-decaying fungi
Call Number
Q183.5.OGC K84 1981
Description.Abstract
The
hydroxylation
and
cleavage
of
conjugated
aryl
olefins
by
horseradish
peroxidase
and
Phanerochaete
chrysosporium
were
investigated
, and
optimum
incubation
conditions
for the
enzyme
reaction
were
developed
.
Substrate
specificity
experiments
showed
that the
enzyme
specificity
corresponded
roughly
to that
exhibited
by the
fungus
, with the
exception
that
P
.
chrysosporium
also
readily
degraded
the
mono-substituted
m-
and
p-methoxycinnamyl
alcohols
to their
corresponding
anisyl
alcohols
. The
pathways
employed
by the
two
systems
were
shown
to be
different
.
[superscript
18]O
tracer
studies
showed
that the
organism
probably
utilized
the
hydroxylation
product
as an
intermediate
,
confirming
earlier
reports
by
other
workers
.
(The
peroxidase
,
however
,
appears
to
cleave
the
olefin
directly
, in
addition
to
catalyzing
the
hydroxylation
reaction
.
It
is
not
able
to
cleave
the
hydroxylated
products.)
Both
peroxidase
and
laccase
purified
from
Polyporus
versicolor
incorporated
labeled
oxygen
only
onto the
B-carbon
of
4-0-
ethylisoeugenol
,
whereas
P
.
chrysosporium
incorporated
a
significant
amount
at the
benzylic
carbon
. In
addition
, the
ability
of the
fungus
to
perform
the
hydroxylation
reaction
in the
presence
of
catalase
suggests
that the
phenol
oxidase(s)
of
P
.
chrysosporium
are not the
sole
catalytic
agent(s)
in the
metabolism
of
lignin-related
aryl
olefins
.
Language
eng
Type
Text
Format.Use
Needs Adobe Acrobat Reader to view.
Format.FileType
pdf
Format.FileSize
1797.752 KB
OCLC number
8886062
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